The bull revoked all the rights of the Jewish community and placed religious and economic restrictions on Jews in the Papal States, renewed anti-Jewish legislation and subjected Jews to various degradations and restrictions on their personal freedom.
The bull established the Roman Ghetto and required the Jews of Rome, who had existed as a community since before Christian times and numbered about 2,000 at the time, to live in it. The Ghetto was a walled quarter with three gates that were locked at night. Under the bull, Jewish males were required to wear a pointed yellow hat, and Jewish females a yellow kerchief. Jews were required to attend compulsory Catholic sermons on the Jewish shabbat.
The bull also subjected Jews to various other restrictions such as a prohibition on property ownership and practising medicine among Christians. Jews were allowed to practice only unskilled jobs, as rag men, secondhand dealers or fish mongers. They could also be pawnbrokers.
Paul IV's successor, Pope Pius IV, enforced the creation of other ghettos in most Italian towns, and his successor, Pope Pius V, recommended them to other bordering states. The Papal States ceased to exist on 20 September 1870 when they were incorporated in the Kingdom of Italy, but the requirement that Jews live in the ghetto was only formally abolished by the Italian state in 1882.
...
Paul IV sought to strictly enforce earlier canonical restrictions against the Jews—as those prohibiting their practising medicine among Christians, employing Christian servants, and the like—but he also restricted them in their commercial activity, forbade them to have more than one synagogue in any city, enforced the wearing of the yellow hat, refused to permit a Jew to be addressed as "signor", and finally decreed that they should live in a designated area separated from Christians. The last measure was carried out in Rome with unrelenting cruelty.
...
The measures were aimed at bringing about Jewish conversions. "These policies were easier to enforce in the Papal States, where the Pope had executive power, as well as elsewhere in Italy, where the papacy had influence. Beyond Italy, though, the provisions of the bull were largely ignored." In Poland, Church officials never proposed segregation of the Jews as such a measure would not have been supported by the king or the nobles.